M, Palma-Vittorelli MB, Palma. Gels with an acrylamide concentration gradient (gradient gels) are also used. Overlay with water to prevent contact with air (oxygen which inhibits polymerization.

In: Electrophoresis of Large DNA Molecules: Theory and Applications, Current Communications in Molecular Biology. San Biagio PL, Madonia F, Newman J, Palma. Use an appropriate gel concentration for your target protein. Richards EG, Lecanidou. Koo HS, Wu HM, Crothers. Stellwagen NC, Magnsdttir S, Gelfi C, Righetti. SDS is a detergent with a strong protein-denaturing effect and binds to the protein backbone at a constant molar ratio. Chiari M, D'Alesio, Consonni R, Righetti. Schägger H, von Jagow.